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人沙眼衣原體抗體酶免試劑盒,(CT)ELISA檢測試劑盒,英文名:CT ELISA Kit,英文縮寫:CT ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人沙眼衣原體抗體ELISA檢測試劑盒,CT 檢測試劑盒,CT 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人沙眼衣原體抗體酶免試劑盒,(CT)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
Ⅰ類主要組織相容性復(fù)合體E(MHCE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Major Histocompatibility Complex Class I E (MHCE)48T/96TY09935Homo sapiens (Human)
谷氨酸受體相互作用蛋白1(GRIP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor Interacting Protein 1 (GRIP1)48T/96TY09936Homo sapiens (Human)
干擾素調(diào)節(jié)因子4(IRF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Regulatory Factor 4 (IRF4)48T/96TY09937Homo sapiens (Human)
表皮轉(zhuǎn)谷氨酰胺酶(TGM3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transglutaminase 3, Epidermal (TGM3)48T/96TY09938Homo sapiens (Human)
肌動蛋白束蛋白(FSCN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fascin (FSCN)48T/96TY09939Homo sapiens (Human)
核糖核酸酶A3(RNASE3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribonuclease A3 (RNASE3)48T/96TY09940Mus musculus (Mouse)
羥甲基戊二酰輔酶A合酶(HMGCS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hydroxymethylglutaryl Coenzyme A Synthase (HMGCS)48T/96TY09941Homo sapiens (Human)
胰腺再生蛋白Iα(REG1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 1 Alpha (REG1a)48T/96TY09942Homo sapiens (Human)
抑瘤素M受體(OSMR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oncostatin M Receptor (OSMR)48T/96TY09943Homo sapiens (Human)
抑制素βB(INHβB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin Beta B (INHbB)48T/96TY09944Homo sapiens (Human)
細(xì)胞色素b-561(CYB561)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome b-561 (CYB561)48T/96TY09945Homo sapiens (Human)
主要穹窿蛋白(MVP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Major Vault Protein (MVP)48T/96TY09946Homo sapiens (Human)
鈣腔蛋白(CALU)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calumenin (CALU)48T/96TY09947Homo sapiens (Human)
淀粉酶α(Amyα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amylase Alpha (Amya)48T/96TY09948Homo sapiens (Human)
白介素2受體α(IL2Rα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 2 Receptor Alpha (IL2Ra)48T/96TY09949Homo sapiens (Human)
毒蕈堿型膽堿受體M3(CHRM3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 3 (CHRM3)48T/96TY09950Homo sapiens (Human)
小乳腺表皮粘蛋白(SBEM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Small Breast Epithelial Mucin (SBEM)48T/96TY09951Homo sapiens (Human)
去氨精氨酸加壓素(DDAVP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 1-Desamino 8D Arginine Vasopressin (DDAVP)48T/96TY09952General
Ⅰ型前膠原氨基端原肽(PⅠNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)48T/96TY09953Rattus norvegicus (Rat)
半胱氨酰白三烯受體2(CYSLTR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cysteinyl Leukotriene Receptor 2 (CYSLTR2)48T/96TY09954Homo sapiens (Human)
絲裂原激活蛋白激酶激酶激酶激酶5(MAP4K5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5)48T/96TY09955Mus musculus (Mouse)
樁蛋白(Pax)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Paxillin (Pax)48T/96TY09956Homo sapiens (Human)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY09957Chicken (Gallus)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY09958Sus scrofa; Porcine (Pig)
巨噬細(xì)胞來源趨化因子(MDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Derived Chemokine (MDC)48T/96TY09959Mus musculus (Mouse)
巨噬細(xì)胞炎性蛋白1α(MIP1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY09960Homo sapiens (Human)
巨噬細(xì)胞炎性蛋白1α(MIP1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY09961Mus musculus (Mouse)
巨噬細(xì)胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY09962Homo sapiens (Human)
巨噬細(xì)胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY09963Mus musculus (Mouse)
胱天蛋白酶11(CASP11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 11 (CASP11)48T/96TY09964Mus musculus (Mouse)
巨噬細(xì)胞炎性蛋白3α(MIP3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY09965Homo sapiens (Human)
巨噬細(xì)胞炎性蛋白3α(MIP3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY09966Mus musculus (Mouse)
巨噬細(xì)胞炎性蛋白3α(MIP3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY09967Rattus norvegicus (Rat)
巨噬細(xì)胞炎性蛋白3β(MIP3β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY09968Homo sapiens (Human)
巨噬細(xì)胞炎性蛋白3β(MIP3β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY09969Mus musculus (Mouse)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 1 (MMP1)48T/96TY09970Homo sapiens (Human)
基質(zhì)金屬蛋白酶10(MMP10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 10 (MMP10)48T/96TY09971Homo sapiens (Human)
基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 13 (MMP13)48T/96TY09972Homo sapiens (Human)
基質(zhì)金屬蛋白酶2(MMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY09973Homo sapiens (Human)
基質(zhì)金屬蛋白酶2(MMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY09974Mus musculus (Mouse)
基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 3 (MMP3)48T/96TY09975Homo sapiens (Human)
基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 3 (MMP3)48T/96TY09976Mus musculus (Mouse)
β-血小板球蛋白(βTG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Thromboglobulin (bTG)48T/96TY09977Homo sapiens (Human)
β-血小板球蛋白(βTG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Thromboglobulin (bTG)48T/96TY09978Rattus norvegicus (Rat)
β-血小板球蛋白(βTG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Thromboglobulin (bTG)48T/96TY09979Mus musculus (Mouse)
維生素E(VE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vitamin E (VE)48T/96TY09980General
組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY09981Sus scrofa; Porcine (Pig)
成纖維細(xì)胞生長因子7(FGF7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 7 (FGF7)48T/96TY09982Sus scrofa; Porcine (Pig)
免疫球蛋白E(IgE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin E (IgE)48T/96TY09983Sus scrofa; Porcine (Pig)
免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY09984Sus scrofa; Porcine (Pig)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。